The Arabidopsis NuA4 histone acetyltransferase complex is required for chlorophyll biosynthesis and photosynthesis

• 作者: Jin-Xing Zhou Xiao-Min Su Si-Yao Zheng Chan-Juan Wu Yin-Na Su Zhaodi Jiang Lin Li She Chen Xin-Jian He
• 来源期刊: 植物学报（英文版）
• 年，卷（期）: 2022,（4）
• 所属期刊栏目: Molecular Physiology
• 页码范围: 901-914
• 页数: 14页
• 语种: 英文

摘要

Although two Enhancer of Polycomb-like proteins, EPL1A and EPL1B (EPL1A/B), are known to be conserved and characteristic subunits of the NuA4-type histone acetyltransferase complex in Arabidopsis thaliana, the biological function of EPL1A/B and the mechanism by which EPL1A/B function in the complex remain unknown. Here, we report that EPL1A/B are required for the histone acetyltransferase activity of the NuA4 complex on the nucleosomal histone H4 in vitro and for the enrichment of histone H4K5 acetylation at thousands of protein-coding genes in vivo. Our results suggest that EPL1A/B are required for linking the NuA4 catalytic subunits HISTONE ACETYLTRANSFERASE OF THE MYST FAMILY 1 (HAM1) and HAM2 with accessory subunits in the NuA4 complex. EPL1A/B function redundantly in regulating plant development especially in chlorophyll biosynthesis and de-etiolation. The EPL1A/B-dependent transcription and H4K5Ac are enriched at genes involved in chlorophyll bio-synthesis and photosynthesis. We also find that EAF6, another characteristic subunit of the NuA4 complex, contributes to de-etiolation. These re-sults suggest that the Arabidopsis NuA4 complex components function as a whole to mediate his-tone acetylation and transcriptional activation specifically at light-responsive genes and are critical for photomorphogenesis.