Theoretical analysis of RNA polymerase fidelity:a steady-state copolymerization approach

• 作者: Wenbo Fu Qiushi Li Yongshun Song Yaogen Shu Zhongcan Ouyang Ming Li
• 来源期刊: 理论物理通讯（英文版）
• 年，卷（期）: 2022,（1）
• 所属期刊栏目: STATISTICAL PHYSICS,SOFT MATTER AND BIOPHYSICS
• 页码范围: 105-114
• 页数: 10页
• 语种: 英文

摘要

The fidelity of DNA transcription catalyzed by RNA polymerase (RNAP) has long been an important issue in biology.Experiments have revealed that RNAP can incorporate matched nucleotides selectively and proofiead the incorporated mismatched nucleotides.However,systematic theoretical researches on RNAP fidelity are still lacking.In the last decade,several theories on RNA transcription have been proposed,but they only handled highly simplified models without considering the high-order neighbor effects and the oligonucleotides cleavage both of which are critical for the overall fidelity.In this paper,we regard RNA transcription as a binary copolymerization process and calculate the transcription fidelity by the steady-state copolymerization theory recently proposed by us for DNA replication.With this theory,the more realistic models considering higher-order neighbor effects,oligonucleotides cleavage,multi-step incorporation and multi-step cleavage can be rigorously handled.