Identification and validation of a major gene for kernel length at the P1 locus in Triticum polonicum

• 作者: Songyue Chai Qin Yao Rui Liu Wenhui Xiang Xue Xiao Xing Fan Jian Zeng Lina Sha Houyang Kang Haiqin Zhang Dan Long Dandan Wu Yonghong Zhou Yi Wang
• 来源期刊: 作物学报（英文版）
• 年，卷（期）: 2022,（2）
• 所属期刊栏目: Research Papers
• 页码范围: 387-396
• 页数: 10页
• 语种: 英文

摘要

Kernel size,one of the traits that determine wheat yield,is controlled by multiple quantitative trait loci.Polish wheat(Triticum polonicum)has elongated and plump kernel and is a valuable material for breeding high-yielding wheat cultivars.However,genes or loci determining kernel length(KL)in Polish wheat are unknown.We identified and validated a major KL gene,KL-PW,at the PI locus in Polish wheat.KL-PW is VRT-A2,which encodes a MIKC-type MADS-box protein(MADS55).An insertion/deletion mutation in intron 1 of VRT-A2PW led to an alternatively spliced transcript,VRT-A2PW2.Quantitative PCR analysis showed that VRT-A2PW was more highly expressed in developing seeds than was VRT-A2Ailanmai.Brassinosteroid(BR)sensitivity experiment and the expression of BR-related genes indicated that VRT-A2PW functions as a positive regulator of BR responses.VRT-A2PW significantly increased KL of wheat.These findings not only reveal the molecular basis of KL-PW in controlling KL,but also provide a valuable genetic resource for increasing kernel size in wheat.