Research on the possible molecular mechanism of Fructus cnidii to improve sleep based on network pharmacology

• 作者: Jin-Liang Guo Ma-Li Feng
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2022,（6）
• 所属期刊栏目: Original article
• 页码范围: 35-40
• 页数: 6页
• 语种: 英文

摘要

Objective: To study the mechanism of cnidium cnidium improving sleep by network pharmacology. Methods: The active components of Fructus cnidii and the targets of active components were obtained by systematic pharmacology database and analysis platform (TCMSP).With the help of Uniprot database;the corresponding target genes of active components were searched, and the targets related to insomnia were retrieved in drugbank and GeneCards databases.Cytoscape 3.7.2 was used to construct the hypnotic active component-insomnia - target network.String database to build PPI network;On the basis of Metascape database, GO enrichment analysis and KEGG enrichment analysis were carried out to predict the mechanism of action. Results: A total of 19 active compounds such as geranyl lignin, osthole, and omellae were screened from Osthole, 55 effective targets were predicted, 962 insomnia related targets were screened, and 27 intersection targets for drug insomnia were made.Among them, PTGS1, PIK3CG, GABRA1, CHRM1, CHRM3, ESR1, etc., may be the key targets of cnidii to improve insomnia. Biological function and pathway enrichment analysis showed that the main active components of Cnidium were involved in chemical synaptic transmission, G-protein-coupled receptor signaling pathway, membrane potential regulation, neurotransmitter transport, monoamine transport, ion transport regulation and other biological processes.Sleep regulation was most likely by regulating Neuroactive ligand-receptor interaction, signaling pathway, Cholinergic synapse, gMP-PKG signaling pathway, it addiction, Serotonergic synapse, and Dopaminergic synapse. Conclusion: The network pharmacology analysis shows that fructus cnidii can improve the characteristics of sleep multi-component, multi-target and multi-pathway, which provides a certain theoretical basis for the research of mechanism of fructus cnidii regulating sleep.