GmNMHC5 may promote nodulation via interaction with GmGAI in soybean

• 作者: Wenting Wang Zhili Wang Wensheng Hou Li Chen Bingjun Jiang Wenya Ma Lijuan Bai Wenwen Song Cailong Xu Tianfu Han Yongjun Feng Cunxiang Wu
• 来源期刊: 作物学报（英文版）
• 年，卷（期）: 2022,（1）
• 所属期刊栏目: Short Communications
• 页码范围: 273-279
• 页数: 7页
• 语种: 英文

摘要

Soybean (Glycine max [L.] Merr.) is a food and oil crop whose growth and yield are influenced by root and nodule development. In the present study, GmNMHC5 was found to promote the formation of nodules in overexpressing mutants. In contrast, the number of nodules in Gmnmhc5 edited with CRISPR/Cas9 decreased sharply. In 35S:GmNMHC5 mutants, expression levels of genes involved in nodulation were significantly up-regulated. Both in vitro and in vivo biochemical analyses showed that GmNMHC5 directly interacted with GmGAI (a DELLA protein), and the content of gibberellin 3 (GA3) in overexpressing mutants was lower than that in the wild type. These results revealed that GmNMHC5 participates in the classical GA signaling pathway, and may regulate the content of GA3 to match the optimal concentra-tion required for nodule formation, thereby promoting nodulation by directly interacting with GmGAI. A model illustrating the mechanism by which GmNMHC5 promotes soybean nodulation is presented.