Enhanced Biodegradation of High-Salinity and Low- Temperature Crude-Oil Wastewater by Immobilized Crude-Oil Biodegrading Microbiota

• 作者: HUANG Xiao ZHOU Ting CHEN Xi BAI Jie ZHAO Yangguo
• 来源期刊: 中国海洋大学学报（自然科学英文版）
• 年，卷（期）: 2022,（1）
• 所属期刊栏目: Marine and Atmospheric Sciences
• 页码范围: 141-151
• 页数: 11页
• 语种: 英文

摘要

High salt and low temperature are the bottlenecks for the remove of oil contaminants by enriched crude-oil degrading microbiota in Liaohe Estuarine Wetland (LEW), China. To improve the performance of crude-oil removal, microbiota was further immobilized by two methods, i.e., sodium alginate (SA), and polyvinyl alcohol and sodium alginate (PVA+SA). Results showed that the crude oil was effectively removed by the enrichment with an average degrading ratio of 19.42–31.45 mg (L d) ?1. The optimal inoculum size for the n-alkanes removal was 10％ and 99.89％. Some members of genera Acinetobacter, Actinophytocola, Aquabac-terium, Dysgonomonas, Frigidibacter, Sphingobium, Serpens, and Pseudomonas dominated in crude-oil degrading microflora. Though the removal efficiency was lower than free bacteria when the temperature was 15℃, SA and PVA+SA immobilization im-proved the resistance to salinity. The composite crude-oil degrading microbiota in this study demonstrated a perspective potential for crude oil removal from surface water under high salinity and low temperature conditions.