Puccinia striiformis Westend.f.sp.tritici(Pst)pathotype CYR34 is widely virulent and prevalent in China.Here,we report identification of a strpie rust resistance(Yr)gene,designated Yr041133,in winter wheat line 041133.This line produced a hypersensitive reaction to CYR34 and conferred resistance to 13 other pathotypes.Resistance to CYR34 in line 041133 was controlled by a single dominant gene.Bulked segre-gant RNA sequencing(BSR-Seq)was performed on a pair of RNA bulks generated by pooling resistant and susceptible recombinant inbred lines.Yr041133 was mapped to a 1.7 cM genetic interval on the chromo-some arm 7BL that corresponded to a 0.8 Mb physical interval(608.9-609.7 Mb)in the Chinese Spring reference genome.Based on its unique physical location Yr041133 differred from the other Yr genes on this chromosome arm.