Development of Ultranarrow-Bore Open Tubular High Efficiency Liquid Chromatography

• 作者: Yingyan Zhou Guangsheng Guo Xiayan Wang
• 来源期刊: 中国化学（英文版）
• 年，卷（期）: 2022,（1）
• 所属期刊栏目: Chemistry Authors Up Close
• 页码范围: 137-152
• 页数: 16页
• 语种: 英文
• DOI: 10.1002/cjoc.202100445

摘要

Chromatography theory shows that when the inner diameter of the chromatography column is very narrow (e.g.,1-2 μm),the maximum efficiency will be produced in liquid chromatography.In addition,the increasing demand for ultrasmall volume sample analysis has produced the trend of nar-rowing the diameter of the liquid chromatography column.Experiments have verified that ultranarrow-bore open tubular liquid chromatography (i.d.≤ 2 μm) has the advantages of small sample requirement and high separation efficiency.However,there also exist huge challenges along with such obvious advantages.This review summarizes the efforts made by our research group and other research groups to develop this field.We hope that in the near future,ultranarrow bore open-tube liquid chromatography can be successfully and maturely applied to the application of analytes with ul-trasmall size and volume such as single-cell and even subcellular organelles omics research.