A pilot-scale multistage constructed wetland-pond (MCWP) system with a "pre-ecological oxidation pond,two-stage horizontal subsurface flow constructed wetland (HSCW) and sur-face flow constructed wetland (SFCW) as the core and postsubmerged plant pond" as the process was used to treat actual polluted river water in the field,and the variation in ni-trogen removal from summer to winter was investigated.The results showed that the av-erage total nitrogen (TN) removal efficiency in the MCWP was approximately 40.74%.The significant positive correlation between the daily highest temperature and the TN removal efficiency of the whole system was fitted with a nonlinear curve (R2 =0.7192).The TN re-moval load rate in the HSCWs was 2.7-3.7 times that in the SFCW.The SFCW,which had high-density plants (35 plants/m2),increased the proportion of nitrogen removed by plant harvesting and microbial function.The TN transformed by Iris pseudacorus L.accounted for 54.53% in the SFCW.Furthermore,bacteria completed the nitrogen cycle in the SFCW through a variety of nitrogen removal pathways.This research not only investigated the TN removal performance in an MCWP system but also made it possible to predict the TN re-moval efficiency according to the daily highest temperature from summer to winter in the field.