The role of chemokines in regualating mast cell recruitment around rat liver tumor

• 作者: ZHANGZhi-Yong RUANYou-Bing
• 来源期刊: 世界今日医学杂志
• 学科: 医学
• 关键词: MCP-1 SCF ELISA
• 年，卷（期）: 2001,（6）
• 页码范围: 483-487
• 页数: 5页
• 分类号: R657

摘要

Aim To explore the correlation of stem cell factor (SCF)and monocyte chemoattractant protein-1 (MCP-1) with number difference of mast cell (MC) around the liver tumor.Methods 40 male wistar rats with liver neoplasm were divided into three different groups by numbers of MC in the surroundings of tumor. We performed ELISA for SCF and MCP-1 in serums. And chemotaxis assays of rat peritoneal MCs to SCF and MCP-1 in serums were measured in 48-wellmicroboyden chambers. We also used immunohistochemistryto investigate whether rat MCs express SCF positively.RESULTE There were marked differences in MC numbersaround tumor between different groups. The group that has more MCs around tumor has the higher levels of SCF andMCP-1 in serums and the stronger chemotaxis to ratperitoneal MCs. SCF washigher than MCP-1 in bothchemotactic activity to MCs and levels in serums. And ratMCs positively express SCF.Conclusion SCF and MCP-1 were found to be two particularly efficacious chemoattractants for MCs. The levels of SCF and MCP-1 in serums may be closely correlated with MCs numbers around tumor. The production of SCF by MCs might act on mast cell migration and proliferation of MC.