Objective To establish the bacterial endotoxin detemination of ciclofalina injection. Methods The cxperiment was implemented according to the request of bacterial endotoxin determination of metronidazole collected by the 2ndbook, CHINA Pharmacopiea, 2000. Results The injection had no interference to the test when it was diluted for 1 times. Thedetecting results of the rabit test was compared with that of the bacterial endotoxin test. Conclusion Amebocye tysele methodcan be used as daily hopsital pyogen test of ciclofalina injection.