SPERM IMMOBILIZATION ACTIVITY OF ALLIUM SATIVUM L. AND OTHER PLANT EXTRACTS

• 来源期刊: 亚洲男性学杂志(英文版)
• 学科: 医学
• 关键词: 口方
• 年，卷（期）: 2003,（2）
• 页码范围: 131-135
• 页数: 5页
• 分类号: R285
• 语种: 中文

摘要

Aim: To identify possible spermicidal agents through screening a number of edible medicinal plants with antimicrobial activity. Methods: Initial screening was made on the basis of ram cauda epididymal sperm immobilization immediately after addition of extracts. The most potent extract was selected and was evaluated on both ram and human spermatozoa. To unravel its mode of action several sperm functional tests were carried out, namely viability of cells, hypo-osmotic swelling test for membrane integrity and assays of membrane-bound enzyme 5'-nucleotidase and acrosomal marker enzyme acrosin. Results: The crude aqueous extract of the bulb of Allium sativum L. showed the most promising results by instant immobilization of the ram epididymal sperm at 0.25 g/mL and human ejaculated sperm at 0.5 g/mL. Sperm immobilizing effects were irreversible and the factor of the extract responsible for immobilization was thermostable up to 90 癈. On boiling at 100 癈 for 10 minutes, this activity was markedly reduced. More