EVALUATION OF DEOXYRIBONUCLEASE ACTIVITY IN SEMINAL PLASMAOF EJACULATED CHICKEN SEMEN

• 来源期刊: 亚洲男性学杂志(英文版)
• 学科: 农学
• 年，卷（期）: 2003,（3）
• 页码范围: 213-216
• 页数: 4页
• 分类号: S852.23
• 语种: 中文

摘要

Aim: To confirm the stability of exogenous genes in the generation of transgenic chickens using ejaculated chicken sperm, the deoxyribonuclease (DNase) activity was evaluated in the seminal plasma of ejaculated semen and the stability of DNA was examined by adding lipofection reagents. Methods: A PCR fragment (249 bp) of pEGFPN-1 vector was used as the DNA substrate and was incubated with the seminal plasma at 40 ℃ for 30 min. Then, the whole reaction solution was subjected to agarose gel electrophoresis and the DNA size was evaluated under UV light. Results: The DNA substrate was completely diminished after incubation with seminal plasma. However, the substrate was intact after incubation with heat-treated seminal plasma or incubation with seminal plasma in the presence of 0.5 mmol/L -5 mmol/L EDTA. The substrate was stabilized in the seminal plasma by the addition of commercially available lipofection reagents. Conclusion: The DNase activity is present in the seminal plasma of ejaculated chicken semen