PERCOLL FRACTIONATION OF ADULT MOUSE SPERMATOGONIA IMPROVESGERM CELL TRANSPLANTATION

• 来源期刊: 亚洲男性学杂志(英文版)
• 学科: 医学
• 年，卷（期）: 2004,（2）
• 页码范围: 93-98
• 页数: 6页
• 分类号: R-332
• 语种: 中文

摘要

Aim: To isolate and transplant germ celis from adult mouse testes for transplantation. Methods: In order to distinguish transplanted celis from endogenous celis of recipients, donor transgenic mice expressing green fluores cent protein (GFP) were used. Germ celis were collected from the donors at 10-12 weeks of age and spermatogonia were concentrated by percoll fractionation and transplanted into recipient seminiferous tubules that had been previ ously treated with busulfan at 5 weeks of age to remove the endogenous spermatogenic celis. Results: Twenty vveeks after the transplantation, a wide spread GFP signal was observed in the recipient seminiferous tubules. The presence of spermatogenesis and spermatozoa was confirmed in sections of 12 out of 14 testes transplanted (86 %). However, when germ celis were transplanted without concentration the success rate was zero (0/9). Conclusion: Germ celis from adult mouse testes can be successfully transplanted into recipient seminiferous tubules if the cell popul