FIBROSIS OF CORPUS CAVERNOSUM IN ANIMALS FOLLOWING CAVERNOUS NERVE ABLATION

• 来源期刊: 亚洲男性学杂志(英文版)
• 学科: 医学
• 年，卷（期）: 2004,（2）
• 页码范围: 111-116
• 页数: 6页
• 分类号: R363
• 语种: 中文

摘要

Aim: To investigate alterations of smooth muscle celis and collagen fibers in corpus cavernosum following cavernous neurectomy and its relation to the expression of transforming growth factor-β1 (TGF-β1). Methods: Ten adult male SD rats (neurectomy group) were subject to a bilateral cavernous nerve (CN) resection aseptically under an operating microscope, with 6 sham-operated rats as the control. Fifteen weeks after the operation, the penile speci mens were collected and prepared for quantitative-analyzing of ratio of smooth muscle to collagen fibers in corpus cavernosum with confocal microscopy, and for detecting the expression of TGF-β1 by RT-PCR and western-blot. Resulte: Smooth muscle celis that show red color after fluorescent-labeling with tetramethylrhodamine isothiocyanate phalloidin and collagen fibers that produce green autofluorescence after paraformaldehyde fixation were clearly iden tified