Bystander Effects on Mammalian Cells Induced by Carbon-ion-radiation

• 作者: Wang Jufang Li Wenjian Dang Bingrong Ma Qiufeng
• 来源期刊: 近代物理研究所和兰州重离子加速器实验室年报：英文版
• 学科: 生物学
• 关键词: 哺乳动物细胞 碳离子辐照 离子辐射 条件培养基 ICM方法 诱导 旁观者效应 V79细胞
• 年，卷（期）: 2004,（1）
• 页码范围: 90
• 页数: 1页
• 分类号: Q78

摘要

<正>Bystander effects on non-irradiated V79 cells were observed by irradiated conditioned medium (ICM) method and co-cultured with carbon-ion-irradiated V79 cells. In the ICM method, cells were irradiated with 1 Gy of carbon ions, cultured with new fresh medium at 37℃for 1 and 2 h. The medium was collected and filtered with 0.22 μm film to remove cells irradiated completely. This medium was transferred into culture dishes with normal cells. After cultured for 7 d, cell colonies were counted. The results were shown in Tab.1, the colony