Irradiation Enhances Transduction of Ad-CMV-p53 into Human Hepatoma Cells

• 作者: Liu Bing Zhang Hong Li Wenjian Hao Jifang Min Fenling Xie Yi Duan Xin Zhou Qingming
• 来源期刊: 近代物理研究所和兰州重离子加速器实验室年报：英文版
• 学科: 生物学
• 关键词: 人肝癌细胞 基因转导 P53 辐射增强 AD CMV 巨细胞病毒 p53基因
• 年，卷（期）: 2004,（1）
• 页码范围: 95
• 页数: 1页
• 分类号: Q691.5

摘要

<正>In order to explore the effects of irradiation-inducing on the transduction of Ad-CMV-p53, human hepatoma (HepG2 line) cells were transinfected with Ad-CMV-p53 after being irradiated with γrays. Survival fractions were measured by Clone Forming. The cell cycle and the expression of p53 were detected by Flow Cytometry (FCM). In certain scope, compared with groups without 60Co γ-rays irradiation, the survival fractions of groups pre-exposed to 60Co γ-rays significantly decreased (Figs.1 and 2). The cycles of cells pre-irradiated by 60Co γ-rays obviously changed (Fig.3). The γirradiation beforehand promoted the transduction and expression of Ad-CMV-p53 (Fig.4).