Irradiation Enhances Transduction of Ad-CMV-p53 into Human Cervix Carcinoma Cell

• 作者: Liu Bing Zhang Hong Li Wenjian Hao Jifang Min Fengling Xie Yi Zhou Qingming Duan Xin
• 来源期刊: 近代物理研究所和兰州重离子加速器实验室年报：英文版
• 学科: 生物学
• 关键词: p53基因 癌细胞 子宫颈 辐射增强 CMV Hela细胞 AD 转导
• 年，卷（期）: 2004,（1）
• 页码范围: 96
• 页数: 1页
• 分类号: Q2

摘要

<正>The human cervix carcinoma (HeLa line) cells pre-irradiated by 60Co γ-rays were transinfected with Ad-CMV-p53. Survival fractions were measured by Clone Forming Unit. Cell cycle and the expression of p53 were detected by Flow Cytometry (FCM). Compared with the groups without pre-irradiation, the killing fractions on Hela cells pre-exposed to 60Co γ-rays significantly increased (Figs.1 and 2) the cycle of Hela cells pre-treated by 60Co γ-rays obviously changed (Fig.3). The pre-exposure of 60Co 7-rays strengthened the expression of p53 (Fig.4).