茶多酚抑制环孢菌素A诱导的慢性肾毒性大鼠转化生长因子-β<em>1</em>的表达

• 作者: Chang-ku JIA Hai-yang XIE Shao-hua SHI You-fa ZHU 郑树森
• 来源期刊: 中国药理学报（英文版）
• 学科: 医学
• 关键词: tea cyclosporine A transforming growth factor-β kidney polymerase chain reaction immunohistochemistry Western blotting
• 年，卷（期）: 2004,（1）
• 所属期刊栏目: 研究原著
• 页码范围: 98-103
• 页数: 6页
• 分类号: R9，R318
• 语种: 英文

摘要

AIM: To investigate the inhibitory effect of tea polyphenols (TP) on the transforming growth factor-β1 (TGF-β1)expression in rat model of cyclosporine A (CsA)-induced chronic nephrotoxicity. METHODS: The rat model of CsA-induced chronic nephrotoxicity was used, 4 groups of rats were respectively treated with vehicle (0.1 mL .kg-1.d-1 At the end of day 28 of treatment, serum and urine are analyzed for creatinine clearance, kidney tissue for pathologic analysis. The TGF-β1 mRNA and its protein expression were detected by RT-PCR, immunohistochemistry,and Western blot. RESULTS: CsA-treated rats had increased renal expression of TGF-βl mRNA and its protein,compared with the vehicle- or TP- treated controls. The renal function and interstitial fibrosis were ameliorated and renal expression of TGF-β1 mRNA and its protein was decreased in animals treated with CsA plus TP, compared with aminals treated with CsA alone (P＜0.05). CONCLUSION: TP significantly inhibits renal expression of TGFβ1 in rat model of cyclosporine-induced chronic nephrotoxicity, suggesting that the decreased renal expression of TGF-β1 exerted by TP is one of mechanisms to protect renal function and tissue structure.