大鼠离体心肌缺血再灌注时内源性血红素氧合酶-1／一氧化碳反应体系的变化

• 作者: CAOLinsheng PENGXiangsheng WANGshiwen
• 来源期刊: 世界急危重病医学杂志
• 学科: 医学
• 关键词: IPC IR HO-1 离体心肌 血红素氧合酶-1 缺血再灌注 大鼠 内源性 RNA CK
• 年，卷（期）: 2005,（2）
• 页码范围: 614-615
• 页数: 2页
• 分类号: R541.4

摘要

Objective To investigate the influence of ischemic reperfusion and ischemic preconditioning on the cardiac structure and function and markers of myocardial injury, especialy on the heine oxygenase-1/carbon monoxide HO-1/CO reactive system. Methods Mode of langendorff was used in isolated rat hearts with retrograde perfusion. Ischemic reperfusion protocol: perfusion for 60 minutes, stopping perfusion for 30 minutes and reperfusion for 30 minutes. Ischemic preconditioning protocol: perfusion for 30 minutes, stopping perfusion for 5 minutes and reperfusion for 5 minutes and repeating three times, stopping perfusion for 30 minutes and reperfusion for 30 minutes. Control protocol: perfusion 120minutes. HO-1 enzyme activity and HbCO levels in myocardium of each group were measured at reperfusion 30minutes. The HO-1 mRNA and protein expression in rat left ventricular myocardium after reperfusion 30 min were examined by RT-PCR and werstern blotting and immunohistochemistry. Indexes of Cardiac Function were recorded in control group, ischemia and reperfusion group(IR) and ischemic preconditioning group (IPC). Indexes of cardiac injury and cardiac oxidative activity were examined. The changes of myocardial structure were checked up by electron microscopy. Results At reperfusion 30min, the indexes of cardiac function in IPC group were significantly higher than that in IR group (P<0.01), but lower than that in control group(P<0.05) and recovery percentage of cardiac function showed the same changes. The activity of HO-1 and the contents of HbCO in myocardium were increased significantly in IR and IPC(P<0.01), but that of IPC were higher (P<0.01). The expression of HO-1 mRNA and Protein in myocardium were also increased significantly in IR and IPC (P<0.01),but that of IPC were higher (P<0.01). The contents of LDH and total CK and MDA were significantly decreased in IPC Compared to IR (P<0.01) and the activity of SOD were increased remarkably in IPC (P<0.01) and there were differnce betwecn IR and IPC (P<0.01). The localizatio