肠球菌体外对人精子膜的影响

• 作者: H.Qiang J.Y.Lin M.S.Jiang W.M.He
• 来源期刊: 亚洲男性学杂志（英文版）
• 年，卷（期）: 2007,（1）
• 所属期刊栏目: 论著
• 页码范围: 77-81
• 页数: 5页
• 语种: 英文

摘要

Aim:To study the influence of enterococci on human sperm membrane in vitro. Methods: Ejaculated human sperm were artificially infected with β-hemolytic or non-β-hemolytic enterococci at the bacteria: sperm ratio of 50:1 at 37℃.Sperm membrane integrity was examined after incubation for 1, 3 and 5 h by hypoosmotic swelling (HOS) test and electron microscopy. Results: Sperm infected with β-hemolytic enterococci had lower HOS scores compared with non-β-hemolytic strains or uninfected control (P ＜ 0.01). The HOS test scores of sperm infected with β-hemolytic enterococci increased in the presence of phosphatidylcholine, an inhibitor of hemolysin. Non-β-hemolytic strains showed no significant difference in swelling rate, compared with the control group (P ＞ 0.05). It was shown by electron microscopy that β-hemolytic enterococci caused significant rupture of human sperm membrane. Conclusion: β-hemolytic enterococci caused human sperm membrane injury, and might be mediated by the hemolysin of enterococci.