Notch1 signaling inhibits growth of EC109 esophageal carcinoma cells through downmodulation of HPV18 E6/E7 gene expression

• 来源期刊: 中国药理学报(英文版)
• 关键词: Notch1 esophageal carcinoma EC109 cell line growth inhibition HPV18 E6/E7 p53
• 年，卷（期）: 2009,（2）
• 页码范围: 153-158
• 页数: 6页
• 语种: 英文
• DOI: 10.1038/aps.2008.16

摘要

Aim:To investigate the role of the Notch1 signaling pathway in growth arrest of an esophageal carcinoma cell line (EC109)in vitro and the mechanism involved.Methods: An intracellular domain of Notch1 (ICN) was transfected into cultured EC109 cells by lipofectamine transfection.Subsequently,the proliferation of the transfected cells was measured by an MTF assay.Cell cycle distribution was ana-lyzed by flow cytometry.Human papillomavirus type 18 (HPV18) E6/E7 mRNA expression was detected by RT-PCR,and p53 protein expression was detected by Western blot.Results: Activation of Notch1 signaling resulted in inhibition of EC109 cell proliferation with the induction of G2/M arrest,downmodulation of HPV18 E6/E7 gene expression,and upregulation of p53 expression.Conclusion: Repression of HPV18 E6/E7 expression by Notch1 signaling results in the activation of p53-mediated pathways with concomitant growth suppression of HPV18-positive EC109 cells.