Contribution of plasma membrane Ca²⁺ ATPase to cerebellar synapse function

• 作者: Helena Huang Molly L Garside Raghavendra Y Nagaraja Ruth M Empson Thomas Knpfel Walther Akemann
• 来源期刊: 世界生物化学杂志：英文版（电子版）
• 学科: 医学
• 关键词: Plasma membrane Ca2+ ATPASE CEREBELLUM Calcium PURKINJE neuron
• 年，卷（期）: 2010,（5）
• 页码范围: 95-102
• 页数: 8页
• 分类号: R338

摘要

The cerebellum expresses one of the highest levels of the plasma membrane Ca2+ATPase,isoform 2 in the mammalian brain.This highly efficient plasma membrane calcium transporter protein is enriched within the main output neurons of the cerebellar cortex;i.e. the Purkinje neurons(PNs) .Here we review recent evidence,including electrophysiological and calcium imaging approaches using the plasma membrane calcium ATPase 2(PMCA2) knockout mouse,to show that PMCA2 is critical for the physiological control of calcium at cerebellar synapses and cerebellar dependent behaviour.These studies have also revealed that deletionof PMCA2 throughout cerebellar development in the PMCA2 knockout mouse leads to permanent signalling and morphological alterations in the PN dendrites. Whilst these findings highlight the importance of PMCA2 during cerebellar synapse function and development,they also reveal some limitations in the use of the PMCA2 knockout mouse and the need for additional experimental approaches including cell-specific and reversible manipulation of PMCAs.