Protective effects of anti-ricin A-chain antibodies delivered intracellularly against ricin-induced cytotoxicity

• 作者: Frank Martiniuk Heinz Kohler Kam-Meng Tchou-Wong Seth Pincus Sybille Müller
• 来源期刊: 世界生物化学杂志：英文版（电子版）
• 学科: 医学
• 关键词: RICIN inhibitors Antibody INTERNALIZATION Cell-penetrating peptide Cell-permeable ANTIBODIES Liposome-encapsulated ANTIBODIES
• 年，卷（期）: 2010,（5）
• 页码范围: 188-195
• 页数: 8页
• 分类号: R392

摘要

AIM:To evaluate the ability of anti-ricin A-chain antibodies,delivered intracellularly,to protect against ricininduced cytotoxicity in RAW264.7 cells. METHODS:Anti-deglycosylated ricin A-chain antibody and RAC18 anti-ricin A-chain monoclonal antibody were delivered intracellularly by encapsulating in liposomes or via conjugation with the cell-penetrating MTS-transport peptide.RAW264.7 cells were incubatedwith these antibodies either before or after ricin exposure.The changes in cytotoxicity were estimated by MTT assay.Co-localization of internalized antibody and ricin was evaluated by fluorescence microscopy. RESULTS:Internalized antibodies significantly increased cell viability either before or after ricin exposure compared to the unconjugated antibodies.Fluorescence microscopy confirmed the co-localization of internalized antibodies and ricin inside the cells. CONCLUSION:Intracellular delivery of antibodies to neutralize the ricin toxin after cellular uptake supports the potential use of cell-permeable antibodies for postexposure treatment of ricin intoxication.