Modulation of protein tyrosine phosphorylation in gastric mucosa during re-epithelization processes

• 作者: Erica K Sloan Kateryna V Lavrova Larysa I Kot Ludmyla I Ostapchenko Olena V Bogdanova Tetyana V Beregova Volodymyr B Bogdanov
• 来源期刊: 世界生物化学杂志：英文版（电子版）
• 学科: 生物学
• 关键词: PROTEIN TYROSINE kinase PROTEIN TYROSINE PHOSPHATASE ANTIOXIDANTS Gastric ULCER WOUND repair
• 年，卷（期）: 2010,（11）
• 页码范围: 338-347
• 页数: 10页
• 分类号: Q51

摘要

AIM:To investigate the role of protein tyrosine phosphorylation in gastric wound formation and repair following ulceration. METHODS:Gastric lesions were induced in rats using restraint cold stress.To investigate the effect of oxidative and nitrosative cell stress on tyrosine phosphorylation during wound repair,total activity of protein tyrosine kinase(PTK),protein tyrosine phosphatase (PTP),antioxidant enzymes,nitric oxide synthase (NOS), 2’,5’-oligoadenylate synthetase,hydroxyl radical and zinc levels were assayed in parallel. RESULTS:Ulcer provocation induced an immediate decrease in tyrosine kinase(40% in plasma membranes and 56% in cytosol,(P【0.05) and phosphatase activity (threefold in plasma membranes and 3.3-fold in cytosol),followed by 2.3-2.4-fold decrease (P【0.05) in protein phosphotyrosine content in the gastric mucosa. Ulceration induced no immediate change in superoxide dismutase (SOD) activity,30% increase (P【0.05) in catalase activity,2.3-fold inhibition (P【0.05) of glutathione peroxidase,3.3-fold increase (P【0.05) in hydroxyl radical content,and 2.3-fold decrease (P【0.05) in zinc level in gastric mucosa.NOS activity was three times higher in gastric mucosa cells after cold stress. Following ulceration,PTK activity increased in plasma membranes and reached a maximum on day 4 after stress (twofold increase,P【0.05),but remained inhibited(1.6-3-fold decrease on days 3,4 and 5,P【0.05) in the cytosol.Tyrosine phosphatases remained inhibited both in membranes and cytosol(1.5-2.4-fold,P【 0.05).NOS activity remained increased on days 1,2 and 3(3.8-,2.6-,2.2-fold,respectively,P【0.05).Activity of SOD increased 1.6 times(P【0.05)days 4 and 5 after stress.Catalase activity normalized after day 2. Glutathione peroxidase activity and zinc level decreased (3.3-and 2-fold,respectively,P【0.05)on the last day. Activity of 2’,5’-oligoadenylate synthethase increased 2.8-fold (P【0.05) at the beginning,and 1.6-2.3-fold (P【0.05) during ulcer recuperation,and normalized on