Mathematical Modeling of the Amperometric Response to Glucose of Glucose Oxidase Films Deposited by AC-Electrophoresis

• 作者: Jan Fransaer Malika Ammam
• 来源期刊: 传感技术(英文)
• 学科: 化学
• 关键词: ALTERNATING Current Electrophoretic Deposition GLUCOSE OXIDASE Modeling
• 年，卷（期）: 2011,（2）
• 页码范围: 17-21
• 页数: 5页
• 分类号: O6

摘要

Previous work illustrated that glucose oxidase (GOx) could be deposited on conducting substrates using asymmetrical alternating current electrophoretic deposition (AC-EPD) to form thick enzyme layers suitable for the manufacturing of highly active biosensors. Here, we modeled the amperometric response of GOx layers to glucose as a function of the thickness of the enzyme layer. The model is based on reaction-diffusion equations with irreversible first-order catalytic reactions. The numerical results displayed qualitative and reasonable quantitative agreement with the experimental data obtained for oxidation currents due to glucose, which increase with the enzyme thickness.