Rhein effects on apoptosis of glomerular mesangial cells cultured with high glucose in Sprague Dawley rats★

• 作者: Fang Hui Guo Yu Li Yan Wei Qun-li Yin Xiao-xing
• 来源期刊: 中国组织工程研究
• 学科: 医学
• 年，卷（期）: 2012,（40）
• 页码范围: 7503-7507
• 分类号: R318
• 语种: 中文
• DOI: 10.3969/j.issn.2095-4344.2012.40.018

摘要

BACKGROUND:Our previous study has already manifested that Chinese medicine Xiaokening containing Rhein can effectively control the development of early diabetic nephropathy. OBJECTIVE:To investigate the effect of Rhein on apoptosis of glomerular mesangial cel s (GMCs) of Sprague Dawley rats cultured with high glucose. METHODS:The GMCs cultured with high glucose were stimulated by different concentrations of Rhein (20,40,80μmol/L). Morphology of GMCs was discriminated by hematoxylin-eosin staining. Karyon apoptosis was examined by fluorescence staining of 4’,6-diamidino-2-phenylindole (DAPI). Cel apoptosis rate was detected by flow cytometry. RESULTS AND CONCLUSION:The outcome of hematoxylin-eosin staining and 4’,6-diamidino-2-phenylindole staining indicated that Rhein could obviously induce apoptosis of GMCs in a dose-and time-dependent manner. The apoptosis rate had significant difference among different groups (P<0.05). This result shows that Rhein can obviously induce apoptosis of GMCs cultured with high glucose and in a dose-and time-dependent manner.