The ATPase activity of molecular chaperone HSP60 is inhibited by immunosuppressant mizoribine

• 作者: Atsushi Komatsuda Fumiko Izuhara Hideaki Itoh Hideki Wakui Kenichi Sawada Masako Tanabe Michiro Otaka Nobuhiro Nakamura Ryuichi Ishida
• 来源期刊: 美国分子生物学期刊(英文)
• 学科: 医学
• 关键词: HSP60 GROEL MIZORIBINE INHIBITION MECHANISMS Conformational Change
• 年，卷（期）: 2012,（2）
• 页码范围: 93-102
• 页数: 10页
• 分类号: R73

摘要

The molecular chaperone HSP60 is a chaperonin homolog of GroEL. We had previously shown that the immunosuppressant mizoribine is bound directly to HSP60 and inhibited its chaperone activity. However, the inhibitory mechanisms of HSP60 by mizoribine have not yet been fully understood. In the present study, we investigated the influence of mizoribine on a folding cycle of HSP60 and co-chaperone HSP10. Our results showed that mizoribine inhibited the folding cycle of HSP60/HSP10. The ATPase activity of HSP60/HSP10 was decreased in the presence of mizoribine and the dissociation of HSP10 from HSP-60 was also decreased by mizoribine. The same functions of GroEL and/or GroES were slightly affected by mizoribine. Based on our findings, we discuss the inhibitory mechanisms of HSP60 by mizoribine.