The restriction endonuclease Tru9I is a useful tool to analyze telomere sequences separately from interstitial telomeric sequences in <i>Arabidopsis thaliana</i>

• 作者: María I. Vaquero-Sedas Miguel A. Vega-Palas
• 来源期刊: 美国分子生物学期刊(英文)
• 学科: 医学
• 关键词: Arabidopsis thaliana TELOMERES ITSS euchromatin HETEROCHROMATIN
• 年，卷（期）: 2012,（3）
• 页码范围: 242-244
• 页数: 3页
• 分类号: R73

摘要

We have previously described a simple method to analyze the chromatin structure of Arabidopsis telomeres independently of that of Interstitial Telomeric Sequences (ITSs). By using this method, we found that, whereas ITSs are heterochromatic, Arabidopsis telomeres exhibit euchromatic features [1]. Some concerns have been recently raised about the accuracy of this procedure [2]. Here, we summarize these concerns and justify our experimental approaches and interpretation of results.