用动态提高对比的 ultrasonography 的 microvascularization 参数大小上的动脉的输入函数的影响

• 作者: Farid Tabarout Ingrid Leguerney Marianne Gauthier Mélanie Polrot Nathalie Lassau Stéphanie Pitre-Champagnat
• 来源期刊: 世界放射学杂志：英文版（电子版）
• 学科: 医学
• 关键词: Dynamic CONTRAST-ENHANCED ULTRASONOGRAPHY ANGIOGENESIS Linear RAW data ARTERIAL input function Functional imaging
• 年，卷（期）: 2012,（7）
• 页码范围: 291-301
• 页数: 11页
• 分类号: R310

摘要

AIM: To evaluate the sources of variation influencing the microvascularization parameters measured by dynamic contrast-enhanced ultrasonography (DCE-US). METHODS: Firstly, we evaluated, in vitro , the impact of the manual repositioning of the ultrasound probe and the variations in flow rates. Experiments were conducted using a custom-made phantom setup simulating a tumor and its associated arterial input. Secondly, we evaluated, in vivo , the impact of multiple contrast agent injections and of examination day, as well as the influence of the size of region of interest (ROI) associated with the arterial input function (AIF). Experiments were conducted on xenografted B16F10 female nude mice. For all of the experiments, an ultrasound scanner along with a linear transducer was used to perform pulse inversion imaging based on linear raw data throughout the experiments. Semi-quantitative and quantitative analyses were performed using two signal-processing methods. RESULTS:In vitro , no microvascularization parameters, whether semi-quantitative or quantitative, were significantly correlated (P values from 0.059 to 0.860) with the repositioning of the probe. In addition, all semiquantitative microvascularization parameters were correlated with the flow variation while only one quantitative parameter, the tumor blood flow, exhibited P value lower than 0.05 (P = 0.004). In vivo , multiple contrast agent injections had no significant impact (P values from 0.060 to 0.885) on microvascularization parameters. In addition, it was demonstrated that semi-quantitative microvascularization parameters were correlated with the tumor growth while among the quantitative parameters, only the tissue blood flow exhibited P value lower than 0.05 (P = 0.015). Based on these results, it was demonstrated that the ROI size of the AIF had significant influence on microvascularization parameters: in the context of larger arterial ROI (from 1.17 ± 0.6 mm 3 to 3.65 ± 0.3 mm 3 ), tumor blood flow and tumor blood volume were correlated with the t