(3-hydroxybutyrate-co-3-hydroxyhexanoate ) Poly 支持间充质的干细胞和 tenocytes 的粘附和移植

• 作者: Alex J Lomas George GQ Chen Alicia J El Haj Nicholas R Forsyth
• 来源期刊: 世界干细胞杂志：英文版（电子版）
• 学科: 医学
• 关键词: MESENCHYMAL stem cell TENOCYTES POLYHYDROXYALKANOATES Hypoxia Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)
• 年，卷（期）: sjgxbzzywbdzb_2012,（9）
• 页码范围: 94-100
• 页数: 7页
• 分类号: R318.08

摘要

AIM: To establish the potential of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) as a material for tendon repair. METHODS: The biocompatibility of PHBHHx with both rat tenocytes (rT) and human mesenchymal stem cells (hMSC) was explored by monitoring adhesive characteristics on films of varying weight/volume ratios coupled to a culture atmosphere of either 21% O2 (air) or 2% O2 (physiological normoxia). The diameter and stiffness of PHBHHx films was established using optical coherence tomography and mechanical testing, respectively. RESULTS: Film thickness correlated directly with weight/volume PHBHHx (r2 = 0.9473) ranging from 0.1 mm (0.8% weight/volume) to 0.19 mm (2.4% weight/volume). Film stiffness on the other hand displayed a biphasic response which increased rapidly at values 】 1.6% weight/volume. Optimal cell attachment of rT required films of ≥ 1.6% and ≥ 2.0% weight/volume PHBHHx in 2% O2 and 21% O2 respectively. A qualitative adhesion increase was noted for hMSC in films ≥ 1.2% weight/volume, becoming significant at 2% weight/volume in 2% O2. An increase in cell adhesion was also noted with ≥ 2% weight/volume PHBHHx in 21% O2. Cell migration into films was not observed. CONCLUSION: This evaluation demonstrates that PHBHHx is a suitable polymer for future cell/polymer replacement strategies in tendon repair.