Seasonal variation of gut Cyanophyta contents and liver GST expression of mud carp(Cirrhina molitorella) and Nile tilapia(Oreochromis niloticus) in the tropical Xiangang Reservoir(Huizhou,China)

• 来源期刊: 科学通报(英文版)
• 学科: 农学
• 年，卷（期）: 2012,（6）
• 页码范围: 616-623
• 页数: 8页
• 分类号: S917.4
• 语种: 中文

摘要

Liver glutathione S-transferase(GST) plays a major role in the detoxification of microcystins(MCs) via conjugation to glutathione(GSH).We evaluated the relationship between seasonal variation in fish gut contents and the expression of GST isoforms in mud carp(Cirrhina molitorella) and Nile tilapia(Oreochromis niloticus).We quantified the abundance and diversity of plankton in the water column and foregut of mud carp and Nile tilapia in the tropical Xiangang Reservoir between October 2007 and July 2008.The mRNA expression of 7 liver GST isoforms was determined by real-time RT-PCR.The gut contents of both species were dependent on the amount and type of phytoplankton and zooplankton in the water.The expression of liver GST genes in Nile tilapia and mud carp was positively and negatively correlated,respectively,with the abundance of toxic cyanobacteria in the fore-gut.The expression of liver GST mRNA was correlated to the abundance of toxic cyanobacteria in the gut contents of both species,suggesting that mRNA expression of GST isoforms could be used as a biomarker in Nile tilapia and mud carp to monitor cyanobacteria blooms in reservoirs.