Expression of matrix metalloproteinases 9 and 12 in actinic cheilitis

• 作者: Anastasios K Markopoulos Athanasios K Poulopoulos Dimitrios Andreadis
• 来源期刊: 世界实验医学杂志
• 学科: 医学
• 关键词: Actinic CHEILITIS Metalloproteinase-9 Metalloproteinase-12 IMMUNOHISTOCHEMISTRY
• 年，卷（期）: 2013,（3）
• 页码范围: 43-49
• 页数: 7页
• 分类号: R

摘要

AIM:To investigate the role of matrix-degrading metalloproteinases 9,12(MMPs),as mediators of functional connective tissue damage in actinic cheilitis.METHODS:Thirty five formalin-fixed,paraffin embedded specimens of actinic cheilitis,and twelve specimens of normal lower lip vermillion,which were obtained by the archives of the Department of Oral Medicine and Maxillofacial Pathology,were examined.From each block,5μm thick sections were cut and routinely stained with Hematoxylin and Eosin.Immunohistochemical studies were performed on 4-μm thick sections of formalin-fixed paraffin embedded actinic cheilitis lesions and of normal lower lip vermillion,for MMP-9 and MMP-12 in serial sections of our specimens.Appropriate positive and negative controls were performed to confirm the specificity of the staining reaction.MMP immunohistochemistry was evaluated using a semiquantitative immunoreactive score.RESULTS:Haematoxylin and eosin staining revealed in actinic cheilitis lesions atrophic stratified squamous cell epithelium,or focally and irregularly hyperplastic of variable thickness,in some areas was observed marked keratin production.Varying degrees of epithelial dysplasia were noticed with a wide spectrum of change within the same specimen.Characteristic was the appearance of chronic inflammatory infiltration,and a band of amorphous acellular,basophilic change like solar elastosis(elastin replacement of collagen).In normal lower lip specimens weak and scanty positive expression of MMP-9 and MMP-12 was observed.Anti-MMP-9 antibody showed a weak reaction,in actinic cheilitis lesions,focal in the elastotic material,in chronic inflammatory cells and mostly in macrophages and neutrophils.Strong and in some cases diffused immunohistochemical expression of MMP-12 was detected in actinic cheilitis lesions in the areas of the fragmented,distorted and thickened elastic fibers.MMP-12 was also expressed in chronic inflammatory cells and mostly macrophages.MMP-12 was significantly higher in actinic cheilitis specimens compared w