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摘要:
A temperature-sensitive (ts) mutant of the CHO-K1 cell line, tsTM18, grows at 340C but not at 390C. Smu1 is the gene responsible for ts defects of tsTM18 cells. Previously, we found that the Smu1 ts defect altered the localization (as indicated by enlargement of speckles) of SRSF1 (SF2/ASF) in tsTM18 cells cultured at 390C, suggesting a functional association between Smu1 and SRSF1. Speckles are subnuclear structures that may function as storage/assembly/ modification compartments to supply splicing factors to active transcription sites. The effect of the ts defect of Smu1 on the localization of other factors related to splicing has not been characterized yet. The mechanisms underlying the enlargement of speckles of SRSF1 remain unclear. In the present study, we found that the ts defect of Smu1 affected the nuclear localization of a splicing factor, SRSF2 (SC35), and factors involved in the exon-exon junction complex, Y14 and ALY. Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that the ts defect of Smu1 affected alternative splicing of endogenous Clk1/ Sty and SRSF2 genes. Mammalian Clk family kinases are shown to phosphorylate serine/arginine (SR) proteins in vitro and SRSF1 in vivo. RT-PCR analysis of Clk1/Sty showed an accumulation of the truncated form lacking kinase activity in tsTM18 cells incubated at 39?C. These data indicate that an accumulation of kinase-negative Clk1/Sty may lead to alteration of the localization of factors related to splicing resulting in the enlargement of speckles.
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篇名 Characterization of the role of Smu1 in nuclear localization of splicing factors in the mammalian temperature-sensitive mutant
来源期刊 美国分子生物学期刊(英文) 学科 医学
关键词 ALTERNATIVE SPLICING PHOSPHORYLATION SPECKLE SPLICING TEMPERATURE-SENSITIVE Mutation
年,卷(期) 2013,(1) 所属期刊栏目
研究方向 页码范围 38-44
页数 7页 分类号 R73
字数 语种
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节点文献
ALTERNATIVE
SPLICING
PHOSPHORYLATION
SPECKLE
SPLICING
TEMPERATURE-SENSITIVE
Mutation
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研究去脉
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期刊影响力
美国分子生物学期刊(英文)
季刊
2161-6620
武汉市江夏区汤逊湖北路38号光谷总部空间
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191
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0
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