A Low Cost and Versatile STED Superresolution Fluorescent Microscope

• 作者: Chin Su Daniel McBride Jun Kameoka Stanislav Vitha
• 来源期刊: 现代仪器(英文)
• 学科: 医学
• 关键词: Stimulated Emission-Depletion MICROSCOPY STED
• 年，卷（期）: 2013,（3）
• 页码范围: 41-48
• 页数: 8页
• 分类号: R73

摘要

A versatile and inexpensive super-resolution fluorescent microscope that functions as easily as a conventional confocal microscope is described. Components of the microscope were designed on a platform which was placed atop a surplus microscope frame. All optical components and equipments used are given. The excitation and depletion beams are extracted from a compact low-cost supercontinuum light source. The focal spot of the depletion beam at the focal plane is studied and imaged by a 100 nm aperture near-field fiber tip. The collinear excitation and depletion beam focused by a 0.9 numerical aperture microscope objective produce a 90 nm lateral super-resolution as verified by imaging 100 nm diameter fluorescent beads.