Purmorphamine Promotes Matrix Mineralization and Cytoskeletal Changes in Human Umbilical Cord Mesenchymal Stem Cells

• 作者: Syed A.Jamal
• 来源期刊: 分子与细胞生物力学(英文)
• 学科: 医学
• 关键词: purmorphamine BMP-2 lithium chloride hUMSCs(human umbilicalcord MESENCHYMAL stem cells FIBROBLAST OSTEOGENIC differentiation cytoskeletons ACTIN stress fibers
• 年，卷（期）: 2013,（4）
• 页码范围: 267-273
• 页数: 7页
• 分类号: R73

摘要

Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) weresubjected to in vitro osteogenic differentiation using a novel combination of signalingmolecules including BMP-2 and purmorphamine. Differentiation outcomeswere assessed by calcein staining and by microscopic examination of the cytoskeleton.Calcein staining showed appreciable degree of calcium mineralization in cellculture, and changes in the morphological attributes of differentiating cells wereobserved vis-a-vis the actin cytoskeleton. Finally, positive calcein staining, alteredcytoskeletal profile, and stress fiber formation in treated cells demonstrated, for thefirst time, a potentially synergistic interplay between BMP-2 and the hedgehog agonist,purmorphamine.This study lends support to the notion of combining small doses of potent moleculesthat can act as safe, less toxic inducers of osteogenic differentiation of human umbilicalcord mesenchymal stem cells with respect to bone regeneration.