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摘要:
Viruses are extremely heterogeneous entities;the size and the nature of their genetic information,as well as the strategies employed to amplify and propagate their genomes,are highly variable.However,as obligatory intracellular parasites,replication of all viruses relies on the host cell.Having co-evolved with their host for several million years,viruses have developed very sophisticated strategies to hijack cellular factors that promote virus uptake,replication,and spread.Identification of host cell factors(HCFs)required for these processes is a major challenge for researchers,but it enables the identification of new,highly selective targets for anti viral therapeutics.To this end,the establishment of platforms enabling genome-wide high-throughput RNA interference(HT-RNAi)screens has led to the identification of several key factors involved in the viral life cycle.A number of genome-wide HT-RNAi screens have been performed for major human pathogens.These studies enable first inter-viral comparisons related to HCF requirements.Although several cellular functions appear to be uniformly required for the life cycle of most viruses tested(such as the proteasome and the Golgi-mediated secretory pathways),some factors,like the lipid kinase Phosphatidylinositol 4-kinaseⅢαin the case of hepatitis C virus,are selectively required for individual viruses.However,despite the amount of data available,we are still far away from a comprehensive understanding of the interplay between viruses and host factors.Major limitations towards this goal are the low sensitivity and specificity of such screens,resulting in limited overlap between different screens performed with the same virus.This review focuses on how statistical and bioinformatic analysis methods applied to HTRNAi screens can help overcoming these issues thus increasing the reliability and impact of such studies.
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篇名 High-throughput RNA interference screens integrative analysis:Towards a comprehensive understanding of the virus-host interplay
来源期刊 世界病毒学杂志 学科 医学
关键词 RNA interference HIGH-THROUGHPUT Cell population Dependency factors Bioinformatics Human immunodeficiency VIRUS Hepatitis C VIRUS DENGUE VIRUS Viral infection Virus-host interactions
年,卷(期) 2013,(2) 所属期刊栏目
研究方向 页码范围 18-31
页数 14页 分类号 R
字数 语种
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研究主题发展历程
节点文献
RNA
interference
HIGH-THROUGHPUT
Cell
population
Dependency
factors
Bioinformatics
Human
immunodeficiency
VIRUS
Hepatitis
C
VIRUS
DENGUE
VIRUS
Viral
infection
Virus-host
interactions
研究起点
研究来源
研究分支
研究去脉
引文网络交叉学科
相关学者/机构
期刊影响力
世界病毒学杂志
不定期
2220-3249
北京市朝阳区东四环中路62号楼远洋国际中
出版文献量(篇)
124
总下载数(次)
0
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