Therapeutic Efficacy of Genistein-Topotecan Combination Compared to Vitamin D3-Topotecan Combination in LNCaP Prostate Cancer Cells

• 作者: Bibiana Sandoval-Bernal James Kumi-Diaka Shreyasee Chakraborty
• 来源期刊: 细胞生物学(英文)
• 学科: 医学
• 关键词: TOPOTECAN GENISTEIN VITAMIN D PROSTATE Cancer
• 年，卷（期）: 2013,（3）
• 页码范围: 97-104
• 页数: 8页
• 分类号: R73

摘要

Background: Prostate cancer is the most common cancer in men over the age of 60 in Western countries. An estimated 241,740 new cases of prostate cancer have been diagnosed in the United States in 2012 with a death toll of 28,170. Varieties of natural phytochemicals such as genistein and topotecan have shown potential chemotherapeutic capacities and are being used to inhibit the growth and proliferation of cell in prostate cancer. Purpose of Study: In this study, we aim to determine the efficacy of Vitamin D3-Topotecan combination compared to Genistein-Topotecan in apoptosis induction in LNCaP prostate cancer cells. Methods: LNCaP cells were grown in complete RPMI medium and cultured at 37°C, 5% CO2 for 23 - 48 hrs to achieve 70% - 80% confluence. The cells were then treated with Genistein-Topotecan, Vitamin D3-Topotecan combination and TPT alone for 24 - 48 hours. In addition, post-treatment assayed using: Trypan Blue exclusion and MTT for cell viability, Ethidium bromide/Acridine orange to determine apoptosis induction, Rhodamine 123/Ethidium bromide to differentiate between viable, apoptotic, and necrotic cells, as well as to assess possible apoptotic mechanism, and DNA fragmentation to discriminate between apoptotic and necrotic cell death. Results: The overall data indicated the dose-and time-dependent cell death in the LNCaP cells and apoptosis as the major mechanism of treatment-induced cell growth arrest. Conclusion: The Genistein-Topotecan combination treatment was significantly more efficacious in growth inhibition of LNCaP cells compared to Vitamin D3-Topotecan or Topotecan alone.