AIM:To investigate the microleakage of two different root canal obturation systems,using the nuclear medicine approach.METHODS:Twenty-six single-rooted extracted teeth were selected.The crowns were sectioned to obtain 15-mm long root segments and each tooth was prepared using rotary ProFile?instruments.The roots were divided into 2 experimental groups using RealSeal 1 and RealSeal sealer or Thermafil and TopSeal sealer as well as two control groups.On the 7th and the 28th day the apices were submersed in a solution of 99mTc-Pertechnetate during 3 h.The radioactivity was counted using aγcamera.RESULTS:The present study showed that none of the root canal-filled teeth was leakage free.The statistical analyses were made using Kruskal-Wallis and statistical significance was assessed usingα=0.05.Although apical leakage measured in counts per minute(cpm)in the Thermafil/TopSeal group was lower than in the RealSeal/RealSeal group(363 916±180 707.7 cpm vs 533 427±414 020.6 cpm)on 7th day and(1 678 200±567 217.4 cpm vs 2 240 518±383 356.7 cpm)on 28th day,there was no statistical difference(P>0.05).In the Thermafil/TopSeal group and RealSeal 1/RealSeal group it was found that over time,the number of counts increased between 7 d and 28 d(363 916±180 707.7 cpm vs 1 678 200±567 217.4 cpm)and(533 427±414 020.6 cpm vs 2 240 518±383 356.7 cpm),respectively,with statistically significant differences(Thermafil/TopSeal group,P=0.015 and RealSeal 1/RealSeal group,P=0.036).CONCLUSION:Both carrier-based Realseal 1 and Thermafil techniques showed a similar sealing effect,but none of the materials was leakage free.