Generation of Unfolded DNA in Human Neutrophils Following Hypothermal Treatment

• 作者: Hisato Saitoh Jin Kawata Makoto Kikuchi
• 来源期刊: 细胞生物学(英文)
• 学科: 医学
• 关键词: Hypothermal TREATMENT DNA UNFOLDING NEUTROPHIL EXTRACELLULAR TRAP (NET)
• 年，卷（期）: 2013,（3）
• 页码范围: 117-124
• 页数: 8页
• 分类号: R73

摘要

By visualizing DNA with diamidino phenylindole (DAPI), we found that hypothermal incubation followed by rewarming of human neutrophils resulted in an increased number of DAPI-positive objects representative of extensive DNA unfolding seemingly similar to neutrophil extracellular traps (NETs). In contrast to canonical NET formation, diphenylene iodonium (DPI), an NADPH oxidase inhibitor, exhibited negligible effects on formation of the DAPI-positive objects. Moreover, multiple instances of DNA damage were detected in the objects, but not in canonical NETs. Our results thus suggest the potential of hypothermia for triggering DNA structural alteration in neutrophils, which is similar to but distinct from NET formation.