Breeding of NHase hyper-producing <i>Rhodococcus ruber</i>strain LUV<sub>30-06</sub>and verification of mutants by RAPD

• 作者: Min Wang Qinghui Wang Shiwei Wang
• 来源期刊: 美国分子生物学期刊(英文)
• 学科: 医学
• 关键词: RHODOCOCCUS ruber NHase MUTAGENESIS Random Amplified POLYMORPHIC DNA
• 年，卷（期）: 2013,（2）
• 页码范围: 108-114
• 页数: 7页
• 分类号: R73

摘要

A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the starting strain CGMCC3090 with ultraviolet irradiation and lithium chloride. The NHase activity of the strain LUV30-06 was increased by 21.99% (3881.3 U/ml), as compared with that of R. ruber CGMCC3090 (3181.4 U/ml). The mutant strain UV30-06 has been proved genetically stable with higher NHase activity in seven successive subcultures as well as random amplified polymorphic DNA analysis (RAPD).