Polyubiquitination and Proteasome Signals in Tubulobulbar Complexes of Rat Late Spermatids

• 作者: Hideaki Fujita Manaka Akashi Sadaki Yokota
• 来源期刊: 细胞生物学(英文)
• 学科: 医学
• 关键词: Tubulobulbar Complex POLYUBIQUITIN SIGNALS PROTEASOME Immunoelectron MICROSCOPY
• 年，卷（期）: 2013,（4）
• 页码范围: 173-178
• 页数: 6页
• 分类号: R73

摘要

To illustrate the involvement of tubulobulbar complexes (TBC) in ubiquitin-proteasome degradation of unnecessary proteins in the head cytoplasm of late spermatids, the localization of polyubiquitin and proteasome was studied by immunofluorescence and immunoelectron microscopy. Polyubiquitin localized to TBC and proteasome subunit α to dense materials surrounding the TBC in the cytoplasm of Sertoli cell enwrapping sickle-shaped spermatid heads. The results suggest that the TBC is a structural device for ubiquin-proteasome degradation of unnecessary proteins in the cytoplasm of spermatid head during rapid reduction of the head cytoplasm and nuclear compaction of late spermatids.