Expression of heat shock protein 70 in rat cornea during wound healing following alkali burn

• 作者: Qin Gang Xu Mei Yu Ling Zhang Xi-bo
• 来源期刊: 中国组织工程研究
• 学科: 医学
• 年，卷（期）: 2012,（37）
• 页码范围: 6973-6978
• 分类号: R318
• 语种: 中文
• DOI: 10.3969/j.issn.2095-4344.2012.37.027

摘要

BACKGROUND:Many factors can influence the wound healing of cornea fol owing alkali burn, heat shock protein can promote the rapidly recovery and clear of the degenerated and injured protein. OBJECTIVE:To investigate whether the expression of heat shock protein 70 after corneal alkali burn in rats is related to corneal wound healing process. METHODS:After the rat eye was checked without inflammation and other lesions, oxybuprocaine eye drops were dropped into the rat eye for twice. Conjunctival sac liquid was sucked by swab, and then the unified specification filter paper of 5 mm diameter was soaked in 1 mol/L NaOH solution for 10 seconds, then the filter paper was placed in the center of the rat cornea for 30 seconds to make the rat corneal alkali burn model. The corneal specimens were obtained at 6 hours,1,3,7,14 and 21 days after alkali burn. RESULTS AND CONCLUSION:Reverse transcription polymerase chain reaction, immunohistochemistry staining and Western blot observation showed that the expression level of heat shock protein 70 increased at 1 day after alkali burn and reached its peak at 7 days. Then, it decreased at 14 days. Hematoxylin-eosin staining and transmission electron microscope observation showed that the corneal injury was most obvious at 6 hours after burning and the wound was gradual y recovered at 7 days after burning. Alkali burned cornea can induce heat shock protein 70 synthesis in a time-dependent manner, also in accordance with the corneal repair procedure. Induced heat shock protein 70 plays an important role in wound healing.