Effect of Hg(II) and Pb(II) Ions on C-Phycocyanin (<i>Spirulina</i><i>platensis</i>)

• 作者: Eteri Gelagutashvili Ketevan Tsakadze
• 来源期刊: 光学与光子学期刊(英文)
• 学科: 医学
• 关键词: C-PHYCOCYANIN HG(II) PB(II) IONS DNA Fluorescence
• 年，卷（期）: 2013,（1）
• 页码范围: 122-127
• 页数: 6页
• 分类号: R73

摘要

Influence of Hg(II) and Pb(II) ions on C-Phycocyanin (C-PC) from cyanobacteria Spirulina platensis was investigated using Fluorescence spectroscopy. Fluorescence measurements demonstrate quenching of C-PC emission by Hg(II) and Pb(II), and blue shifts in the fluorescence spectra. The effect of DNA on the fluorescence of Hg(II)-and Pb(II)-C-PC (from Spirulina platensis) complexes was also studied. It was shown that the fluorescence intensity of Hg-C-PC after addition of DNA gave rise to the fluorescence buildup. At the same time, addition of DNA to the Pb(II)-C-PC complexes showed no such effect. In the case of Hg(II)-C-PC, fluorescence intensity significantly decreases in time, while for Pb(II)-C-PC, decrease of the fluorescence intensity is not significant, but blue shift of the peak takes place.