基本信息来源于合作网站,原文需代理用户跳转至来源网站获取       
摘要:
Gastric parietal cells are important in acid secretion, but it is unclear which cells throughout the gastric gland have the highest secretion potency. Here, we used immunohistochemical methods with anti-H+, K+-ATPase, phosphoryl ezrin and CD44 antibodies to study the distribution of gastric acid secretion activity. Stomach tissues from freely fed and starved rats were cryofixed for light microscopy or fixed by high-pressure freezing for electron microscopy. Parietal cells from freely fed animals corresponded to the active secretion phase and to the inactive resting phase from starved rats. Anti-H+, K+-ATPase and anti-phosphoryl ezrin labeling were observed on the membrane of the intracellular canaliculi and the tubulovesicle from freely fed rats, while cells from starved animals showed weak labeling with anti-phosphoryl ezrin antibody staining. Morphometrical analysis at the electron microscopic level was performed on active and inactive acid secretory phases between the upper and base regions of the gland. H+, K+-ATPase and CD44 were distributed on both sites of the microvillous and tubulovesicle membrane in the same cells, but phosphoryl ezrin localized predominantly on the microvillous membrane in active cells of the glandular neck and upper base. Therefore, the highest secreting potency appeared to be in cells of the glandular neck and upper base.
推荐文章
Rock chemical weathering by sulfuric acid: pathway, method and prospect
Chemical weathering
Sulfuric acid
Global climate change
Sulfate isotope
Isotherm and kinetic studies on the adsorption of humic acid molecular size fractions onto clay mine
Kaolinite
Montmorillonite
Leonardite humic acid
Humic acid fractions
Kinetics
Equilibrium
Hydrogeochemical processes and multivariate analysis for groundwater quality in the arid Maadher reg
Groundwater quality
Hydrogeochemical processes
Multivariate analysis
Salinity
Mio-Plio
Quaternary aquifer
Groundwater quality assessment using multivariate analysis, geostatistical modeling, and water quali
Groundwater
Multivariate analysis
Geostatistical modeling
Geochemical modeling
Mineralization
Ordinary Kriging
内容分析
关键词云
关键词热度
相关文献总数  
(/次)
(/年)
文献信息
篇名 Immunohistochemical Analysis of the Acid Secretion Potency in Gastric Parietal Cells
来源期刊 细胞生物学(英文) 学科 医学
关键词 Gastric PARIETAL Cells SECRETORY POTENCY PHOSPHORYL Ezrin HISTOCHEMICAL MORPHOMETRY
年,卷(期) 2013,(4) 所属期刊栏目
研究方向 页码范围 179-185
页数 7页 分类号 R73
字数 语种
DOI
五维指标
传播情况
(/次)
(/年)
引文网络
引文网络
二级参考文献  (0)
共引文献  (0)
参考文献  (0)
节点文献
引证文献  (0)
同被引文献  (0)
二级引证文献  (0)
2013(0)
  • 参考文献(0)
  • 二级参考文献(0)
  • 引证文献(0)
  • 二级引证文献(0)
研究主题发展历程
节点文献
Gastric
PARIETAL
Cells
SECRETORY
POTENCY
PHOSPHORYL
Ezrin
HISTOCHEMICAL
MORPHOMETRY
研究起点
研究来源
研究分支
研究去脉
引文网络交叉学科
相关学者/机构
期刊影响力
细胞生物学(英文)
季刊
2325-7776
武汉市江夏区汤逊湖北路38号光谷总部空间
出版文献量(篇)
71
总下载数(次)
0
论文1v1指导