Enhancement of Ethanol Production from Napiergrass (<i>Pennisetum purpureum</i>Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment

• 作者: Hayato Nagai Kazuyoshi Ohta Keisuke Takeo Masahide Yasuda Takuya Uto Tomoko Matsumoto Toshifumi Yui Yasuyuki Ishii
• 来源期刊: 可持续生物质能源系统(英文)
• 学科: 医学
• 关键词: LMAA SSF Cellulase XYLANASE SACCHAROMYCES CEREVISIAE Escherichia coli KO11
• 年，卷（期）: 2013,（3）
• 页码范围: 179-185
• 页数: 7页
• 分类号: R73

摘要

Napiegrass (Pennisetum purpureum Schumach) was treated with a low-moisture anhydrous ammonia (LMAA) pretreatment by adding an equal weight of water and keeping it under atmospheric ammonia gas at room temperature for four weeks. After the removal of ammonia and washing with water, a simultaneous saccharification and fermentation (SSF) was conducted for the LMAA-pretreated napiergrass (1.33 g) in a buffer solution (8 mL) using a mixture of a cellulase (80 mg) and a xylanase (53 mg) as well as the cell suspension (0.16 mL) of Saccharomyces cerevisiae. Ethanol and xylose resulted in 91.2% and 62.9% yields, respectively. The SSF process was scaled up using LMAA-pretreated napiergrass (100.0 g) to give ethanol (77.2%) and xylose (52.8%). After the removal of ethanol, the pentose fermentation of the SSF solution (40 mL), which contained 1.00 g of xylose, using cell suspension of Escherichia coli KO11 (70 mL) gave 86.3% yield of ethanol. Total ethanol yield reached 68.9% based on xylan (21.4 wt%) and glucan (39.7 wt%) of the LMAA-pretreated napiergrass.