Isolation and expression analysis of CBF4 from <i>Vitis amurensis</i>associated with stress

• 作者: Bailin Wang Chang Dong Jianmin Tao Junpeng Ren Lizhen Xiao Meng Zhang Yang Qin Zhen Zhang Zhiying Yu
• 来源期刊: 农业科学(英文)
• 学科: 医学
• 关键词: VITIS amurensis CBF4 STRESS TRANSCRIPT Accumulation
• 年，卷（期）: 2013,（5）
• 页码范围: 224-229
• 页数: 6页
• 分类号: R73

摘要

The C-repeat Binding Factor/Dehydration Responsive Element Binding factor (CBF/DREB) responsive pathway is involved in plant response to abiotic stress. In this study, we report the isolation of VaCBF4 (a complete cDNA) and its promoter from Vitis amurensis through rapid amplification of cDNA ends and genome walking techniques, respectively. The CBF4 transcript accumulation of V. amurensis increased under cold, salinity, and abscisic acid (ABA) and salicylic acid (SA) treatments, whereas that of Vitis vinifera showed a different change. The transcript levels of VaCBF4 inthe roots, stems, leaves, and petioles under cold, salinity, and ABA and SA treatments were up-regulated in a timedependent manner. The presence of the cis-elements MBC, ABRE, and as-2-box in the VaCBF4 promoter further confirmed that this promoter is a component of the CBF transduction pathway, which is involved in plant response to multistress.