Biological Characteristics of Fusariumoxysporum f. sp. niveum Isolated from Watermelon Cultivated in Plastic Greenhouse and Indoor Toxicity Determination of Different Fungicides in Hainan Province of China

• 作者: Fan Hongyan
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 农学
• 关键词: 室内毒力测定 生物学特性 塑料大棚 西瓜汁 海南省 杀菌剂 SP 多菌灵可湿性粉剂
• 年，卷（期）: pdapzwbchyj_2013,（5）
• 页码范围: 1-5
• 页数: 5页
• 分类号: S436.5

摘要

[Objective]The paper was to study biological characteristics of Fusarium oxysporum f.sp.niveum in watermelon cultivated in plastic greenhouse in Hainan Province of China and to determine indoor toxicity of fungicides.[Method]Effects of various factors on mycelial growth and spore germination were studied,and the lethal temperature of spores and indoor toxicity of fungicides were also determined.[Result]The fungal mycelium had a better growth rate on watermelon juice medium than the others.The optimum temperature for mycelial growth and spore germination were 28℃and 30℃,and the optimum pH values were7-8 and 7-9,respectively.Light-dark alternation was conducive for mycelial growth.The lethal temperature of spore was 60℃for 5 min.The fungal mycelium grew better while taking fructose and glucose as carbon sources and yeast extract and peptone as nitrogen sources.Toxicity determination showed that 50%prochloraz-manganese chloride complex WP had the greatest inhibition effect on mycelial growth,with the EC50value of 0.730 9μg/mL;followed by 32.5%benzoic azoxystrobin SC,30%benzoylate·propiconazol EC,10%difenoconazole WG,25%bromothalonil·carbendazim WP and 25%bromothalonil WP,with the EC50values from 1.884 7μg/mL to 8.161 0μg/mL.[Conclusion]The study provided basic data for field control against F.oxysporum f.sp.niveum on watermelon cultivated in plastic greenhouse in Hainan Province.