Development of <i>in vitro</i>culture of rat Leydig cells after purification with Nycodenz gradient

• 作者: Ekayanti Mulyawati Kaiin Ita Djuwita Mohamad Agus Setiadi Tuty Laswardi Yusuf
• 来源期刊: 动物科学期刊(英文)
• 学科: 医学
• 关键词: LEYDIG In VITRO RAT Nycodenz hCG ITS TESTOSTERONE
• 年，卷（期）: 2013,（4）
• 页码范围: 299-304
• 页数: 6页
• 分类号: R73

摘要

The study examined the effect of human Chorionic Gonadotrophin (hCG) and/or Insulin Transferring Sodium Selenite (ITS) on the proliferation and development of rat Leydig cells purified by Nycodenz gradient. Leydig cells purity, viability and proliferation after purification and 3 days of cultured were evaluated. Leydig cells 1 × 106 cells/ml were cultured in DMEM containing 10% Newborn Calf Serum (NBCS) and divided into four kinds of treatments 1) as a control, 2) control + 2.5 IU/ml hCG, 3) control + ITS (5 μg/ml insulin, 10 μg/ml transferrin, 5 μg/ml Se), and 4) control + hCG + ITS. Leydig cells purification results showed 91.40% of purity, viability was 98.17% and concentration 7.03 × 106 cells/ml. The addition of ITS and hCG + ITS in DMEM produced Leydig cell proliferation by 88.35% and 90.64% higher than in controls (86.82%) (p p p