Rapid <i>In-Vitro</i>and <i>In-Vitro</i>Detection of <i>Chalara fraxinea</i>by Means of Mass Spectrometric Techniques

• 作者: Eberhard Krause Heike Stephanowitz Irmtraut Zaspel Michael Schuemann Thi Lam Huong Pham
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: Chalara fraxinea RAPID In-Vitro In-Vitro DETECTION MS TECHNIQUES MALDI LC-MS
• 年，卷（期）: mgzwxqkyw,（2）
• 页码范围: 444-453
• 页数: 10页
• 分类号: R73

摘要

For the first time, mass spectrometric (MS) techniques were employed to rapidly detect the pathogen Chalara fraxinea in-vitro and directly in-vivo in tissues of diseased ash trees caused by C. fraxinea, using a range of characteristic novel secondary metabolites of C. fraxinea as chemical markers for the presence of the pathogen. We have found an evident correlation between the presence and amount of these-only for C. fraxinea characteristic and novel-secondary metabolites (named chalarafraxinines) and the degree of disease of respective infected ash seedlings. As demonstrated in this work, the MS based high-throughput-screening approach constitute an alternative to the time consuming and expensive micro biological isolation procedures for detection of the pathogen C. fraxinea and furthermore, can be used to rapidly test ash genotypes for resistance / susceptibility to C. fraxinea infection.